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Evidence Guide: MSL973020 - Perform histological procedures

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL973020 - Perform histological procedures

What evidence can you provide to prove your understanding of each of the following citeria?

Process tissue

  1. Perform pre-use checks to ensure processor is fit for purpose
  2. Select program and reagents for processing
  3. Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement
  4. Check processor for errors that occurred during processing sequence
Perform pre-use checks to ensure processor is fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select program and reagents for processing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check processor for errors that occurred during processing sequence

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue

  1. Check that volume of wax is sufficient for uninterrupted embedding of processor load
  2. Check that temperature of wax is suitable for embedding process
  3. Embed tissue in correct orientation
  4. Apply procedures to prevent cross-contamination between tissues
  5. Inspect blocks, reject and re-embed items that do not meet quality control standards
Check that volume of wax is sufficient for uninterrupted embedding of processor load

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check that temperature of wax is suitable for embedding process

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue in correct orientation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain sections

  1. Select reagents specified in the method
  2. Stain sections according to the method
  3. Examine sections microscopically to ensure expected staining outcomes have been achieved
  4. Mount sections to ensure long-term preservation
  5. Attach permanent labels giving specimen details according to workplace traceability requirements
Select reagents specified in the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain sections according to the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine sections microscopically to ensure expected staining outcomes have been achieved

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Mount sections to ensure long-term preservation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Attach permanent labels giving specimen details according to workplace traceability requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select program and reagents for processing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check processor for errors that occurred during processing sequence

  1. Check that volume of wax is sufficient for uninterrupted embedding of processor load
Check that volume of wax is sufficient for uninterrupted embedding of processor load

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check that temperature of wax is suitable for embedding process

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue in correct orientation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut sections of tissue

  1. Label required number of microscope slides in accordance with workplace traceability requirements
Label required number of microscope slides in accordance with workplace traceability requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check the flotation bath is ready and satisfactory for use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut representative sections at the required thickness observing prescribed safety measures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Float sections onto water bath to flatten tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Pick up sections onto microscope slides ensuring identification on slides matches that on the block

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

  1. Select reagents specified in the method
Select reagents specified in the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain sections according to the method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine sections microscopically to ensure expected staining outcomes have been achieved

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Mount sections to ensure long-term preservation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Attach permanent labels giving specimen details according to workplace traceability requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Ensure personal safety and minimise cross-contamination through the use of personal protective equipment (PPE)
Ensure personal safety and minimise cross-contamination through the use of personal protective equipment (PPE)

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Handle chemicals and sharps safely

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge